Single Molecule Studies of Genome Packaging in Bacteriophage Phi29 Reveal Nonequilibrium DNA Dynamics and Continuous Allosteric Regulation of the Packaging Motor Complex
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Copyright Information: All rights reserved unless otherwise indicated. Contact the author or original publisher for any necessary permissions. eScholarship is not the copyright owner for deposited works. Learn more at iii The dissertation of Zachary T. Berndsen is approved, and it is acceptable in quality and form for publication on microfilm: 2015 iv DEDICATION I dedicate this work to my family in recognition of their unconditional support.
منابع مشابه
Translocation of nicked but not gapped DNA by the packaging motor of bacteriophage phi29.
The biomolecular mechanism that the double-stranded DNA viruses employ to insert and package their genomic DNA into a preformed procapsid is still elusive. To better characterize this process, we investigated packaging of bacteriophage phi29 DNA with structural alterations. phi29 DNA was modified in vitro by nicking at random sites with DNase I, or at specific sites with nicking enzyme N.BbvC I...
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تاریخ انتشار 2015